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Gradient shape and precolumn volume %b 0 5 10 15 20 25 30 minutes theoretical gradient no column, 1. In addition to providing an introduction to hplc for pharmaceutical analysis it is intended that this book will be a useful resource. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Impresoras hp como imprimir a doble cara impresion duplex.